Phenotype and Virulence Assessment of a Burkholderia pseudomallei Soil Isolate from Malaysia

Burkholderia pseudomallei, a Gram-negative soil saprophyte, is the causative agent of life-threatening melioidosis. B. pseudomallei from and water remains common source human animal infection via skin abrasions, ingestion or inhalation. Despite reported sero-prevalence in healthy individuals among Malaysian rice farmers, there are limited reports on isolated around country. In this study, we characterized isolate compared it to local clinical isolates. 16s rRNA sequencing was adopted confirm identity isolate, NC20. NC20 colony morphology, vitro growth rate antibiotic sensitivity were examined two isolates, UM6 D286. Virulence properties such as biofilm formation nematode host also examined. The exhibited distinguishable features comparable similar resistance profile Additionally, medium-level producer with levels D286, where amount produced much less relative UM6. Interestingly, weaker killing Caenorhabditis elegans model comparison between soil-derived isolates demonstrated that both shared certain phenotypic but somewhat virulent than used study.